Neurogenesis during caudal spinal cord regeneration in adult newts

Dev Genes Evol. 1999 Jun;209(6):363-9. doi: 10.1007/s004270050265.

Abstract

After tail amputation in urodele amphibians, dramatic changes appear in the spinal cord rostral to the amputation level. Transection induces a proliferation response in cells lining the ependymal canal, giving rise to an ependymal tube in which neurogenesis occurs. Using the thymidine analog bromodeoxyuridine (BrdU) in short- and long-term labeling of cells undergoing DNA synthesis (S phase of the cell cycle), specific cell markers, and cell cultures, we show that neurons derive from the proliferative ependymal layer of the ependymal tube.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Division
  • Cells, Cultured
  • Central Nervous System / growth & development*
  • Glial Fibrillary Acidic Protein / analysis
  • Immunohistochemistry
  • Neuroglia / chemistry
  • Neuroglia / cytology
  • Neuroglia / physiology
  • Neurons / chemistry
  • Neurons / cytology
  • Neurons / physiology
  • Phosphopyruvate Hydratase / analysis
  • Pleurodeles / growth & development
  • Pleurodeles / physiology*
  • Regeneration*
  • Spinal Cord / cytology
  • Spinal Cord / physiology*
  • Spinal Cord / ultrastructure
  • Tail

Substances

  • Glial Fibrillary Acidic Protein
  • Phosphopyruvate Hydratase