Engraftment and multilineage expression of human bone marrow CD34- cells in vivo

Ann N Y Acad Sci. 1999 Apr 30:872:220-31; discussion 231-2. doi: 10.1111/j.1749-6632.1999.tb08467.x.

Abstract

The fetal sheep competitive engraftment model of human hematopoietic stem cells (HSC) was used to evaluate the in vivo engraftment potential of human bone marrow CD34- Lin- cells. Transplantation of CD34- Lin- cells into primary hosts resulted in the long-term (> 1 year) engraftment and multilineage donor cell/progenitor expression with production of significant numbers of CD34+ cells. Secondary transplantation and limiting dilution studies confirmed the presence in human CD34- fraction of HSC with in vivo long-term engraftment and multilineage differentiation potentials.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD / analysis
  • Antigens, CD34 / analysis*
  • Biomarkers / analysis
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / immunology
  • Cells, Cultured
  • Fetus
  • Graft Survival / physiology*
  • Hematopoietic Stem Cell Transplantation*
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Sheep
  • Transplantation, Heterologous / immunology
  • Transplantation, Heterologous / physiology*

Substances

  • Antigens, CD
  • Antigens, CD34
  • Biomarkers