Zebrafish genetic map with 2000 microsatellite markers

Genomics. 1999 Jun 15;58(3):219-32. doi: 10.1006/geno.1999.5824.


The zebrafish is the first vertebrate organism used for large-scale genetic screens seeking genes critical to development. These screens have been quite successful, with more than 1800 recessive mutations discovered that speak to morphogenesis of the vertebrate embryo. The cloning of the mutant genes depends on a dense genetic map. The 2000 markers we present here, using microsatellite (CA) repeats, provides 1.2-cM average resolution. One centimorgan in zebrafish is about 0. 74 megabase, so, for many mutations, these markers are close enough to begin positional cloning by YAC walks.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cloning, Molecular
  • Female
  • Gene Library
  • Genetic Linkage
  • Genome
  • Male
  • Microsatellite Repeats / genetics*
  • Mutation
  • Physical Chromosome Mapping
  • Zebrafish / genetics*