Inhibitory effect of resveratrol on interleukin 6 release by stimulated peritoneal macrophages of mice

Phytomedicine. 1999 May;6(2):79-84. doi: 10.1016/S0944-7113(99)80039-7.


In the present investigation, interleukin 6 (IL-6) activity in the supernatant of cultured mouse peritoneal macrophages was monitored using a sensitive bioassay involving the IL-6-dependent murine hybridoma B9 cell line. The effects of resveratrol on Il-6 release by mouse peritoneal macrophages stimulated with calcium ionophore A23187 and fMLP were explored. Resveratrol, at a concentration range from 5 x 10(-6) to 4 x 10(-5) mol.l-1, was found to dose-dependently inhibit IL-6 release by cultured macrophages induced by A23187 and fMLP, and showed no direct cytotoxic effect, but induced proliferation of cultured mouse thymus cells. Resveratrol, at a concentration range from 10(-8) to 10(-5) mol.l-1, was shown to dose-dependently inhibit calcium ion influx into the cells with the stimulation of fMLP (10(-6) mol.l-1). These results suggest that the blocking of calcium ion influx into cells by reveratrol is one of the possible mechanisms of the IL-6 biosynthesis inhibitory action of resveratrol.

MeSH terms

  • Animals
  • Calcimycin / pharmacology
  • Calcium / metabolism
  • Cells, Cultured
  • Female
  • Interleukin-6 / antagonists & inhibitors*
  • Interleukin-6 / metabolism
  • Ion Transport
  • Macrophage Activation / drug effects
  • Macrophages, Peritoneal / drug effects*
  • Macrophages, Peritoneal / metabolism
  • Mice
  • Mice, Inbred C57BL
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / drug effects
  • Neutrophils / metabolism
  • Rats
  • Resveratrol
  • Stilbenes / pharmacology*
  • Thymus Gland / cytology
  • Thymus Gland / drug effects
  • Thymus Gland / metabolism


  • Interleukin-6
  • Stilbenes
  • Calcimycin
  • N-Formylmethionine Leucyl-Phenylalanine
  • Resveratrol
  • Calcium