[A study of a derivative of Taq DNA polymerase]

Yi Chuan Xue Bao. 1999;26(2):179-85.
[Article in Chinese]


Using the method of double primer oligonucleotide-mediated mutagenesis, the high expression plasmid of TaqND236, a derivative of Taq DNA polymerase, was constructed. To determine the frameshift mutation frequency of the in vitro DNA synthesis, we constructed a Gapped-DNA system using the pFDPM118 (a mutant of pUC118 with a -1 frameshift mutation on the lacZ gene) as template. By calculating the ratio of blue and white colonies on the X-gal plate after transforming E. coli TG1, the frameshift mutation frequency of Taq and TaqND236 was measured. It was found that the replication fidelity of the deleted Taq-TaqND236 increased more than 10 folds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Replication
  • Escherichia coli / genetics
  • Frameshift Mutation
  • Taq Polymerase / genetics
  • Taq Polymerase / metabolism*


  • Taq Polymerase