Characterization of an Arabidopsis thaliana receptor-like protein kinase gene activated by oxidative stress and pathogen attack

Plant J. 1999 May;18(3):321-7. doi: 10.1046/j.1365-313x.1999.00447.x.


An Arabidopsis thaliana cDNA clone that encodes a putative receptor-like protein kinase gene (At-RLK3) was characterized. The deduced 667-amino acid protein consists of an amino-terminal signal sequence, an extracellular domain, a single transmembrane domain, and a cytoplasmic domain with characteristics of serine/threonine protein kinase. Because of the original features of its extracellular domain, the At-RLK3 protein is a member of a new class of receptor-like protein kinases. The At-RLK3 gene is present as a single copy within the Arabidopsis genome and its transcripts are detected in root, stem, leaf and flower. In cultured cells, the At-RLK3 gene is activated upon oxidative stress and salicylic acid treatment. In plants, the gene appears to be differentially regulated during various plant-pathogen interactions: upon inoculation with strains of Pseudomonas syringae pv. tomato harboring or not, different avr genes, At-RLK3 transcripts accumulate transiently at similar levels during both compatible and incompatible interactions. This gene is, however, preferentially expressed during the incompatible interaction induced by the soil-borne vascular bacteria, Ralstonia solanacearum. The involvement of At-RLK3 in signal transduction pathways during pathogen attack is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics*
  • Arabidopsis / microbiology
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • DNA, Plant / genetics
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Plant / drug effects
  • Genes, Plant*
  • Molecular Sequence Data
  • Oxidative Stress
  • Protein Kinases / genetics*
  • Pseudomonas / pathogenicity
  • Salicylic Acid / pharmacology
  • Sequence Homology, Amino Acid
  • Tissue Distribution


  • DNA Primers
  • DNA, Complementary
  • DNA, Plant
  • Protein Kinases
  • Salicylic Acid

Associated data

  • GENBANK/AJ011674
  • GENBANK/L00670
  • GENBANK/L22302