Expression and localization of p80 and p68 interleukin-1 receptor proteins in the brain of adult mice

J Neuroimmunol. 1999 Jan 1;93(1-2):194-202. doi: 10.1016/s0165-5728(98)00224-0.


The biological effects of interleukin-1 (IL-1) are mediated by two distinct receptors, the p80 type I IL-1 and p68 type II IL-1 receptor proteins (IL-1RI and IL-1RII, respectively), both of which have been recently co-localized to the growth hormone synthesizing cells of the adenohypophysis. Previous studies have shown that IL-1 can bind to specific structures in the central nervous system, but the distribution of IL-1RI and IL-1RII proteins in the adult mouse brain has not been reported. Here we have used immunohistochemistry to study the expression, distribution and cellular localization of both isoforms of the IL-1 receptor proteins in the adult mouse brain. Using a combination of processing techniques (AMeX fixation and cryosectioning), we have immunolabeled brain sections for each isoform of the IL-1R. Both isoforms are expressed in the CNS, particularly in neuronal soma of the granular layer of the dentate gyrus and pyramidal cells of fields CA1-CA4 of Ammon's horn of the hippocampus, in epithelial cells of the choroid plexus and ependymal layer, and in neuronal soma of Purkinje cells of the cerebellum. The IL-1RII isoform, but not IL-1RI, is expressed in specific neuronal soma and proximal cell processes of neurons of the paraventricular gray matter of the hypothalamus. These immunohistochemical data directly demonstrate the neuronal expression of both IL-1R proteins in situ. The distribution and cellular localization of IL-1R proteins in the CNS provide a molecular basis for understanding reciprocal interactions between the immune system and the brain.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Age Factors
  • Animals
  • Brain Chemistry / immunology*
  • Choroid Plexus / chemistry
  • Choroid Plexus / immunology
  • Ependyma / chemistry
  • Ependyma / immunology
  • Female
  • Hippocampus / chemistry
  • Hippocampus / immunology
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred Strains
  • Molecular Weight
  • Neuritis / immunology
  • Neuritis / metabolism
  • Receptors, Interleukin-1 / analysis*
  • Receptors, Interleukin-1 / biosynthesis
  • Receptors, Interleukin-1 / chemistry
  • Receptors, Interleukin-1 Type I
  • Receptors, Interleukin-1 Type II


  • Receptors, Interleukin-1
  • Receptors, Interleukin-1 Type I
  • Receptors, Interleukin-1 Type II