Inhibitory effect of mast cell-mediated immediate-type allergic reactions by Cichorium intybus

Pharmacol Res. 1999 Jul;40(1):61-5. doi: 10.1006/phrs.1999.0474.

Abstract

We investigated the effect of an aqueous extract of Cichorium intybus (CIAE) on mast cell-mediated immediate type allergic reactions. CIAE (0.1-1000 mg kg-1) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. Especially, CIAE inhibited compound 48/80-induced anaphylactic reaction 100% with the dose of 1000 mg kg-1. CIAE 1000 mg kg-1also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with CIAE at a concentration ranging from 0.1 to 1000 mg kg-1, the plasma histamine levels were reduced in a dose-dependent manner. CIAE (1-1000 microg ml-1) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMC, when CIAE (1000 microg ml-1) was added, increased significantly compared with that of control cells. These results indicate that CIAE inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphylaxis / chemically induced
  • Anaphylaxis / prevention & control
  • Animals
  • Anti-Allergic Agents / therapeutic use*
  • Chicory / chemistry
  • Chicory / therapeutic use*
  • Cyclic AMP / metabolism
  • Histamine Release / drug effects
  • Hypersensitivity, Immediate / drug therapy*
  • Mast Cells / drug effects*
  • Mast Cells / physiology
  • Mice
  • Mice, Inbred BALB C
  • Passive Cutaneous Anaphylaxis / drug effects*
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Wistar
  • p-Methoxy-N-methylphenethylamine

Substances

  • Anti-Allergic Agents
  • Plant Extracts
  • p-Methoxy-N-methylphenethylamine
  • Cyclic AMP