Rescue of cyclin D1 deficiency by knockin cyclin E

Cell. 1999 Jun 11;97(6):767-77. doi: 10.1016/s0092-8674(00)80788-6.


D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene (Ccnd1) have been deleted and replaced by those of human cyclin E (CCNE). In the tissues and cells of these mice, the expression pattern of human cyclin E faithfully reproduces that normally associated with mouse cyclin D1. The replacement of cyclin D1 with cyclin E rescues all phenotypic manifestations of cyclin D1 deficiency and restores normal development in cyclin D1-dependent tissues. Thus, cyclin E can functionally replace cyclin D1. Our analyses suggest that cyclin E is the major downstream target of cyclin D1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cyclin D1 / deficiency
  • Cyclin D1 / genetics
  • Cyclin D1 / metabolism*
  • Cyclin E / genetics
  • Cyclin E / metabolism*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Phenotype
  • Receptors, Estrogen / metabolism
  • Retina / metabolism


  • Cyclin E
  • Receptors, Estrogen
  • Cyclin D1