Multilocus sequence typing: molecular typing of bacterial pathogens in an era of rapid DNA sequencing and the internet

Curr Opin Microbiol. 1999 Jun;2(3):312-6. doi: 10.1016/S1369-5274(99)80054-X.


Multilocus sequence typing is a development of multilocus enzyme electrophoresis in which the alleles at multiple house-keeping loci are assigned directly by nucleotide sequencing, rather than indirectly from the electrophoretic mobilities of their gene products. A major advantage of this approach is that sequence data are unambiguous and electronically portable, allowing molecular typing of bacterial pathogens (or other infectious agents) via the Internet.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacteria / classification
  • Bacteria / genetics
  • Bacterial Typing Techniques*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Genes, Bacterial / genetics*
  • Humans
  • Internet
  • Sequence Analysis, DNA


  • DNA, Bacterial