Ribosomal DNA sequencing as a tool for identification of bacterial pathogens

Curr Opin Microbiol. 1999 Jun;2(3):299-305. doi: 10.1016/S1369-5274(99)80052-6.


Conventional methods for the identification and characterization of clinical isolates of bacterial pathogens sometimes fall short when such isolates exhibit unusual phenotypic profiles. Recent advances in DNA sequencing technology have greatly enhanced the ability of the microbiologist to determine the identity of a bacterial isolate. Given the relative objectivity of DNA sequence information and growing availability of sequence information databases, a significant movement is now afoot to use molecular methods for the identification of clinical pathogens.

Publication types

  • Review

MeSH terms

  • Bacteria / chemistry
  • Bacteria / classification
  • Bacteria / genetics*
  • Base Sequence
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics*
  • Humans
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA*


  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S