Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products

Curr Opin Microbiol. 1999 Jun;2(3):323-7. doi: 10.1016/S1369-5274(99)80056-3.


Terminal restriction fragment length polymorphism is a recent molecular approach that can assess subtle genetic differences between strains as well as provide insight into the structure and function of microbial communities. The technique has both high sensitivity and throughput making it ideal for comparative analyses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Ecosystem
  • Genetic Variation
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length*
  • RNA, Ribosomal, 16S / classification
  • RNA, Ribosomal, 16S / genetics*


  • RNA, Ribosomal, 16S