DGGE/TGGE a Method for Identifying Genes From Natural Ecosystems

Curr Opin Microbiol. 1999 Jun;2(3):317-22. doi: 10.1016/S1369-5274(99)80055-1.

Abstract

Five years after the introduction of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in environmental microbiology these techniques are now routinely used in many microbiological laboratories worldwide as molecular tools to compare the diversity of microbial communities and to monitor population dynamics. Recent advances in these techniques have demonstrated their importance in microbial ecology.

Publication types

  • Review

MeSH terms

  • Ecosystem*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Environmental Microbiology
  • Genes / genetics*
  • RNA, Ribosomal, 16S / genetics

Substances

  • RNA, Ribosomal, 16S