Characterization of eae+ Escherichia coli isolated from healthy and diarrheic calves

Vet Microbiol. 1999 May;66(4):251-63. doi: 10.1016/s0378-1135(99)00013-9.

Abstract

Strains of Escherichia coli from 101 healthy and 114 diarrheic calves were screened by PCR for the eae (intimin) gene and Shiga toxin genes (stx). Each eae+ and eae/stx+ strain was examined for antimicrobial susceptibility, enterohemolysin activity, and the somatic O antigen was determined. An immunoassay was used to detect Shiga toxin antigens for the eae/stx+ E. coli. Significantly more (p = 0.005) of the healthy calves carried eae+ and eae/stx+ E. coli in their feces when compared to strains from diarrheic calves. Moreover, Shiga toxin antigens were detected significantly more (p = 0.001) often among the eae/stx+ strains from healthy calves when compared to eae/stx+ strains from diarrheic calves. However, significantly more (p = 0.001) of the eae+ and eae/stx+ strains from diarrheic calves were resistant to at least one of the antimicrobials tested, and the strains from diarrheic calves had a significantly (p = 0.05) higher rate of antimicrobial resistance to at least two different antimicrobial classes. No significant difference (p> or =0.05) was detected among the eae+ and eae/stx+ strains from healthy and diarrheic calves for enterohemolysin production. Serogroups O-negative, O5, O26, and O111 were predominate among both healthy and diarrheic calves.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesins, Bacterial*
  • Agglutination Tests / veterinary
  • Animals
  • Antigens, Bacterial / analysis
  • Bacterial Outer Membrane Proteins / chemistry
  • Bacterial Outer Membrane Proteins / genetics*
  • Bacterial Toxins / chemistry
  • Bacterial Toxins / genetics
  • Carrier Proteins*
  • Cattle
  • Cattle Diseases / microbiology*
  • DNA Primers / chemistry
  • DNA, Bacterial / chemistry
  • Diarrhea / microbiology
  • Diarrhea / veterinary*
  • Drug Resistance, Microbial
  • Electrophoresis, Agar Gel / veterinary
  • Enterotoxins / chemistry
  • Enterotoxins / genetics
  • Escherichia coli / classification
  • Escherichia coli / genetics
  • Escherichia coli / pathogenicity*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Escherichia coli Proteins*
  • Feces / microbiology
  • Female
  • Hemolysin Proteins / analysis
  • Immunoenzyme Techniques / veterinary
  • Intestines / pathology
  • Michigan
  • Polymerase Chain Reaction / veterinary
  • Shiga Toxins

Substances

  • Adhesins, Bacterial
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Toxins
  • Carrier Proteins
  • DNA Primers
  • DNA, Bacterial
  • Enterotoxins
  • Escherichia coli Proteins
  • Hemolysin Proteins
  • Shiga Toxins
  • eaeA protein, E coli