Genomic organization of three neurotoxins active on small conductance Ca2+-activated potassium channels from the scorpion Buthus martensi Karsch

FEBS Lett. 1999 Jun 11;452(3):360-4. doi: 10.1016/s0014-5793(99)00651-1.

Abstract

According to the known primary sequences of three neurotoxins active on small conductance Ca2+-activated potassium channels from the scorpion Buthus martensi Karsch, their corresponding cDNAs were cloned and sequenced using 3'- and 5'-RACE. All of them encoded a signal peptide composed of 28 residues and a mature toxin of 29, 28 and 33 residues, respectively. Their cDNA deduced sequences were totally consistent with those determined, and the C-terminal amidation of one neurotoxin was confirmed. The genomic DNAs of these three toxins were also amplified by PCR, cloned and sequenced. They all consisted of two exons disrupted by a small single intron. All of these introns were inserted within the signal peptide at the same -10 position upstream from the mature toxin, consisting of 94, 78 and 87 bp, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Cloning, Molecular
  • DNA, Complementary
  • Exons
  • Introns
  • Molecular Sequence Data
  • Neurotoxins / chemistry
  • Neurotoxins / genetics*
  • Neurotoxins / toxicity
  • Polymerase Chain Reaction
  • Potassium Channels / adverse effects*
  • Protein Sorting Signals / genetics
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / toxicity
  • Scorpion Venoms / chemistry
  • Scorpion Venoms / genetics*
  • Scorpion Venoms / toxicity
  • Scorpions / genetics*

Substances

  • BmP01 toxin
  • BmP03 toxin
  • BmP05 toxin
  • DNA, Complementary
  • Neurotoxins
  • Potassium Channels
  • Protein Sorting Signals
  • Recombinant Proteins
  • Scorpion Venoms

Associated data

  • GENBANK/AF095780
  • GENBANK/AF095781
  • GENBANK/AF097408