In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

FEBS Lett. 1999 Jun 11;452(3):365-70. doi: 10.1016/s0014-5793(99)00648-1.

Abstract

The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Cellulase / genetics
  • Cellulose 1,4-beta-Cellobiosidase
  • Cloning, Molecular
  • Humans
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • N-Acetylglucosaminyltransferases / genetics
  • N-Acetylglucosaminyltransferases / metabolism*
  • Oligosaccharides / chemistry
  • Polysaccharides / biosynthesis*
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification
  • Promoter Regions, Genetic
  • Recombinant Proteins / metabolism
  • Spores, Fungal
  • Trichoderma / metabolism
  • Trichoderma / physiology*

Substances

  • Oligosaccharides
  • Polysaccharides
  • Recombinant Proteins
  • N-Acetylglucosaminyltransferases
  • alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I
  • Cellulase
  • Cellulose 1,4-beta-Cellobiosidase