Abstract
The human N-acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2 was obtained by NMR analysis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbohydrate Sequence
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Cellulase / genetics
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Cellulose 1,4-beta-Cellobiosidase
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Cloning, Molecular
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Humans
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Magnetic Resonance Spectroscopy
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Molecular Sequence Data
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N-Acetylglucosaminyltransferases / genetics
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N-Acetylglucosaminyltransferases / metabolism*
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Oligosaccharides / chemistry
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Polysaccharides / biosynthesis*
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Polysaccharides / chemistry
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Polysaccharides / isolation & purification
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Promoter Regions, Genetic
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Recombinant Proteins / metabolism
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Spores, Fungal
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Trichoderma / metabolism
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Trichoderma / physiology*
Substances
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Oligosaccharides
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Polysaccharides
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Recombinant Proteins
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N-Acetylglucosaminyltransferases
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alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I
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Cellulase
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Cellulose 1,4-beta-Cellobiosidase