Vascular endothelial growth factor-C expression in human prostatic carcinoma and its relationship to lymph node metastasis

Br J Cancer. 1999 Apr;80(1-2):309-13. doi: 10.1038/sj.bjc.6690356.


Lymph node dissemination is a major prognostic factor in human cancer. However, the molecular mechanisms underlying lymph node metastasis are poorly understood. Recently, vascular endothelial growth factor-C (VEGF-C) was identified as a ligand for VEGF receptor-3 (VEGFR-3/Flt-4) and the expression of VEGFR-3 was found to be highly restricted to the lymphatic endothelial cells. In this report, we investigated the expression of VEGF-C and VEGFR-3 in human prostatic carcinoma tissue by using in situ hybridization and immunohistochemical staining respectively. Expression of VEGF-C mRNA in prostatic carcinoma was significantly higher in lymph node-positive group than in lymph node-negative group. In addition, the number of VEGFR-3-positive vessels was increased in stroma surrounding VEGF-C-positive prostatic carcinoma cells. These results suggest that the expression of VEGF-C in prostatic carcinoma cells is implicated in the lymph node metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / metabolism*
  • Adenocarcinoma / pathology
  • Endothelial Growth Factors / biosynthesis*
  • Humans
  • In Situ Hybridization
  • Lymphatic Metastasis*
  • Male
  • Neoplasm Metastasis
  • Prostatic Neoplasms / metabolism*
  • Prostatic Neoplasms / pathology
  • RNA, Messenger / analysis
  • RNA, Neoplasm / analysis
  • Tumor Cells, Cultured
  • Vascular Endothelial Growth Factor C


  • Endothelial Growth Factors
  • RNA, Messenger
  • RNA, Neoplasm
  • Vascular Endothelial Growth Factor C