Decreased glomerular proteinase activity in the streptozotocin diabetic rat

Am J Nephrol. 1999;19(3):441-6. doi: 10.1159/000013492.


Decreased glomerular proteinase activity may contribute to matrix accumulation in diabetes. Male Sprague-Dawley rats were rendered diabetic by injection of streptozotocin (STZ) 65 mg/kg i.v.; age-matched, sham-injected rats served as controls. Glomeruli from diabetic rats 1 month after STZ injection demonstrated significant decreases in collagenase and cathepsin B activities compared to control glomeruli. Treatment with insulin resulted in a slight (but not significant) increase in collagenase activity and normalized cathepsin B activity. We conclude that decreased glomerular collagenase and cathepsin B activities are present in STZ diabetes. These alterations may contribute to mesangial matrix accumulation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cathepsin B / metabolism*
  • Collagenases / metabolism*
  • Diabetes Mellitus, Experimental / enzymology*
  • Kidney Glomerulus / enzymology*
  • Male
  • Rats
  • Rats, Sprague-Dawley


  • Cathepsin B
  • Collagenases