High resolution multicolor-banding: a new technique for refined FISH analysis of human chromosomes

Cytogenet Cell Genet. 1999;84(3-4):156-60. doi: 10.1159/000015245.


A new multicolor-banding technique has been developed which allows the differentiation of chromosome region specific areas at the band level. This technique is based on the use of differently labeled overlapping microdissection libraries. The changing fluorescence intensity ratios along the chromosomes are used to assign different pseudo-colors to specific chromosome regions. The multicolor banding of human chromosome 5 is presented as an example.

Publication types

  • Comparative Study

MeSH terms

  • Chromosome Banding / methods*
  • Chromosome Deletion
  • Chromosome Painting / methods*
  • Chromosomes, Human / genetics*
  • Chromosomes, Human, Pair 2 / genetics
  • Chromosomes, Human, Pair 5 / genetics
  • DNA Probes / genetics
  • Fluorescent Dyes
  • Genomic Library
  • Humans
  • Neoplasms / genetics
  • Reproducibility of Results
  • Sensitivity and Specificity


  • DNA Probes
  • Fluorescent Dyes