The nuclear pore complex: from molecular architecture to functional dynamics

Curr Opin Cell Biol. 1999 Jun;11(3):391-401. doi: 10.1016/S0955-0674(99)80055-6.


Toward dissecting the molecular composition and architecture of the nuclear pore complex (NPC), over the past 18 months novel nucleoporins and NPC subcomplexes were identified and characterized. The three-dimensional structure of isolated yeast NPCs was determined by electron cryomicroscopy. New specimen preparation and labeling protocols localized a number of nucleoporins and NPC subcomplexes within the three-dimensional architecture of the yeast NPC. Structural changes of native NPCs mediated by physiological effectors such as calcium or ATP were monitored by time-lapse atomic force microscopy, thus revealing a first glimpse of the NPC's functional dynamics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Humans
  • Membrane Glycoproteins / physiology
  • Nuclear Envelope / physiology*
  • Nuclear Envelope / ultrastructure
  • Nuclear Proteins / physiology


  • Membrane Glycoproteins
  • Nuclear Proteins