Cloning and chromosomal mapping of the human DNA polymerase theta (POLQ), the eighth human DNA polymerase

Genomics. 1999 Jul 1;59(1):90-6. doi: 10.1006/geno.1999.5843.

Abstract

We have cloned the cDNA for the eighth human DNA polymerase, DNA polymerase θ. The human cDNA encodes a putative DNA polymerase of 1762 amino acids with a calculated molecular mass of 198 kDa. The derived protein sequence is homologous to the Drosophila melanogaster mus308 protein product, a putative DNA polymerase-helicase involved in repair of interstrand crosslinks. The C-terminal region contains the canonical DNA polymerase motifs A, B, and C found in the family A type of DNA polymerases, which includes Escherichia coli polymerase I. The N-terminal region contains a putative ATP binding domain but not motifs for a helicase. The gene was mapped by radiation hybrid analysis to chromosome 3q within an interval flanked by proximal marker D3S1303 and distal marker D3S3576 and, based on proximity to a gene that has been mapped cytogenetically, within band 3q13.31.

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3 / genetics
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA-Directed DNA Polymerase / genetics*
  • Genetic Markers
  • HeLa Cells
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured

Substances

  • DNA, Complementary
  • Genetic Markers
  • DNA polymerase theta
  • DNA-Directed DNA Polymerase

Associated data

  • GENBANK/AF052573