Remarkable increase in cerebrospinal fluid 3-nitrotyrosine in patients with sporadic amyotrophic lateral sclerosis

Ann Neurol. 1999 Jul;46(1):129-31. doi: 10.1002/1531-8249(199907)46:1<129::aid-ana21>;2-y.


To investigate the significance of peroxynitrite-mediated oxidative damage in the pathogenesis of sporadic amyotrophic lateral sclerosis (SALS), the concentrations of 3-nitrotyrosine and tyrosine in the cerebrospinal fluid (CSF) of patients with SALS were determined. The concentration of 3-nitrotyrosine and the 3-nitrotyrosine/ tyrosine ratio in patients with SALS were approximately seven times those of controls. Thus, the present findings in living patients provide in vivo evidence for a possible role of peroxynitrite, a mediator of oxidative stress, and increased nitration of tyrosine residues in the pathogenesis of SALS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Amyotrophic Lateral Sclerosis / cerebrospinal fluid*
  • Chromatography, High Pressure Liquid
  • Female
  • Humans
  • Male
  • Middle Aged
  • Tyrosine / analogs & derivatives*
  • Tyrosine / cerebrospinal fluid


  • 3-nitrotyrosine
  • Tyrosine