Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells

Int J Oncol. 1999 Aug;15(2):229-35. doi: 10.3892/ijo.15.2.229.


We examined the susceptibility of six human gastric carcinoma cell lines to infection with recombinant p53 adenovirus vector (AxCA-p53). AxCA-p53 infection at a muliplicity of infection (MOI) of 50 resulted in apoptotic cell death (MKN-1 cells), growth arrest (MKN-45, MKN-74 and KATO-III cells), or non-effectiveness (TMK-1 and OCUM-2M cells). Western blot analysis revealed increasing expression levels of p21/WAF1 protein after infection with AxCA-p53 in all the cell lines. After infection with AxCA-p53, the expression levels of bax or bcl-XL protein changed in MKN-1, but not in the other cell lines. These results suggest that the apoptotic pathway (dependence on the expressions of bcl-2 family proteins) dominates the growth arrest pathway (dependence on the expressions of p21/WAF1 protein) after infection with AxCA-p53. Thus, the bcl-2 family might play a crucial role in p53-mediated growth arrest and apoptosis in human gastric carcinoma cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Apoptosis / physiology*
  • Cell Cycle / physiology
  • Cell Division / physiology
  • DNA Fragmentation
  • Gene Transfer Techniques*
  • Genes, p53*
  • Genetic Vectors*
  • Humans
  • Proto-Oncogene Proteins c-bcl-2 / biosynthesis
  • Recombination, Genetic
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / therapy*
  • Tumor Cells, Cultured


  • Proto-Oncogene Proteins c-bcl-2