Cloning of a novel kinase (SIK) of the SNF1/AMPK family from high salt diet-treated rat adrenal

FEBS Lett. 1999 Jun 18;453(1-2):135-9. doi: 10.1016/s0014-5793(99)00708-5.


PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the SNF1/AMPK family was isolated. An in vitro kinase assay demonstrated that SIK protein had autophosphorylation activity. Northern blot revealed that SIK mRNA levels were markedly augmented by ACTH treatment both in rat adrenal glands and in Y1 cells. SIK may play an important role in the regulation of adrenocortical functions in response to high plasma salt and ACTH stimulation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Cortex / cytology
  • Adrenal Cortex / drug effects
  • Adrenal Cortex Neoplasms
  • Adrenal Glands / enzymology*
  • Adrenocorticotropic Hormone / pharmacology
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression / drug effects
  • Molecular Sequence Data
  • Potassium, Dietary / pharmacology*
  • Protein Kinases / genetics
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sodium Chloride, Dietary / pharmacology*
  • Tumor Cells, Cultured


  • Potassium, Dietary
  • Sodium Chloride, Dietary
  • Adrenocorticotropic Hormone
  • Protein Kinases
  • AMP-activated protein kinase kinase
  • SNF1-related protein kinases
  • Protein-Serine-Threonine Kinases
  • Sik1 protein, rat

Associated data

  • GENBANK/AB020480