Identification of a novel gene, DAM1, amplified at chromosome 1p13.3-21 region in human breast cancer cell lines

Cancer Lett. 1999 Jun 1;140(1-2):219-26. doi: 10.1016/s0304-3835(99)00091-9.

Abstract

Screening for differentially expressed genes in cancer cell lines by the RNA differential display (DD) technique identified a novel mRNA that encodes a 26-kDa protein and is up-regulated in MCF-7 and BT-20 breast cancer cells. The predicted amino acid sequence showed 38% homology to Caenorhabditis elegans T12A2.7 protein, indicating that this is a possible human homologue for C. elegans T12A2.7 protein. The mRNA, designated DAM1 (DNA amplified in mammary carcinoma), was mapped at the 1p13.3-21 region, which is frequently altered in human breast cancer. By Southern blot analysis, we confirmed that the up-regulation of this novel gene is associated with gene amplification in MCF-7 (10-fold) and BT-20 (5-fold) cells. Our findings suggest that the DAM1 gene is a novel gene up-regulated by amplification in human breast cancer cell lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Breast Neoplasms / genetics*
  • Caenorhabditis elegans / genetics
  • Carcinoma / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1 / genetics
  • Cloning, Molecular
  • Female
  • Genes, Neoplasm*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Tumor Cells, Cultured

Substances

  • BCAS2 protein, human
  • Neoplasm Proteins

Associated data

  • GENBANK/AB020623