The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH)

Biochem Biophys Res Commun. 1999 Jul 5;260(2):416-25. doi: 10.1006/bbrc.1999.0865.


The human adipocyte-specific apM-1 gene encodes a secretory protein of the adipose tissue that has been suggested to play a role in the pathogenesis of obesity. The regulation of apM-1 was studied along adipocyte differentiation. While apM-1-mRNA and apM-1 protein were absent in preadipocytes and in 48 h differentiated adipocytes, they were found upregulated from day 4 to day 9 of adipocyte differentiation as shown by RNase protection assay and Western blot analysis. These data indicate that apM-1 may be a late marker of adipocyte differentiation. In human sera apM-1 protein is also detectable by Western blots using a polyclonal antibody raised against a synthetic peptide sequence of the human apM-1. The genomic structure of the human apM-1 gene together with a total of 2.7 kb of the 5'-flanking region with putative transcription factor binding sites is presented. Interestingly, sequence comparisons link the apM-1 gene to the family of TNF's and to genes expressed in activated T-cells. The chromosomal localization of apM-1 was investigated by FISH and mapped to human chromosome 1q21.3-1q23, a region that was identified as a susceptibility locus for Familial Combined Hyperlipidaemia (FCH) and polygenic NIDDM. These data and the chromosomal localization on chromosome 1q21.3-q23 raises the possibility that apM-1 as an adipocyte-specific secretory protein may play a role in the pathogenesis of FCH and associated insulin resistance. Exon- and intron-specific primer sequences are presented as a basis for mutation screening of patients affected with FCH.

MeSH terms

  • 3T3 Cells
  • Adiponectin
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • Exons
  • Genetic Linkage
  • Genetic Predisposition to Disease*
  • Humans
  • Hyperlipidemia, Familial Combined / genetics*
  • In Situ Hybridization, Fluorescence
  • Intercellular Signaling Peptides and Proteins*
  • Introns
  • Lymphocyte Activation
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Proteins / genetics*
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid
  • T-Lymphocytes / metabolism*
  • Tumor Necrosis Factor-alpha / genetics*


  • Adiponectin
  • Intercellular Signaling Peptides and Proteins
  • Proteins
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha

Associated data

  • GENBANK/AJ131459
  • GENBANK/AJ131460
  • GENBANK/AJ131461
  • GENBANK/AJ131462
  • GENBANK/AJ131463