Abstract
Tumor-associated carbohydrate (TAC) antigens are important targets in cancer vaccine efforts. Carbohydrates are, however, frequently poor immunogens, in that they are T-cell-independent antigens. Molecular mimicry of TAC by peptides is an alternative approach to generating anti-carbohydrate immune responses. Here we demonstrate that peptide mimotopes can elicit antibody responses that cross-react with representative human TAC antigens. Primary immunization with such a multiple antigenic peptide, along with QS-21 as adjuvant, elicits cytotoxic antibodies reactive with naturally occurring forms of TAC expressed on tumor cells, and vaccination of mice with peptide mimotopes reduced tumor growth and prolonged host survival in a murine tumor model.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies / blood
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Antigens, Tumor-Associated, Carbohydrate / immunology*
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Cancer Vaccines / immunology*
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Cancer Vaccines / therapeutic use
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Complement System Proteins / immunology
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Cytotoxicity, Immunologic
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Flow Cytometry
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Humans
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Lewis Blood Group Antigens / immunology
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Mice
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Mice, Inbred BALB C
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Molecular Mimicry*
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Molecular Sequence Data
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Peptides / chemical synthesis
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Peptides / chemistry
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Peptides / immunology*
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Sarcoma, Experimental / chemically induced
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Sarcoma, Experimental / immunology*
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Sarcoma, Experimental / therapy
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Tumor Cells, Cultured
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Vaccination*
Substances
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Antibodies
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Antigens, Tumor-Associated, Carbohydrate
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Cancer Vaccines
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Lewis Blood Group Antigens
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Peptides
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peptide 106
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Complement System Proteins