High mobility group-like protein in bovine milk stimulates the proliferation of osteoblastic MC3T3-E1 cells

Biochem Biophys Res Commun. 1999 Jul 22;261(1):113-7. doi: 10.1006/bbrc.1999.0871.


The active component in bovine milk on the proliferation of osteoblastic MC3T3-E1 cells was purified and identified. Growth-promoting activity was measured by [(3)H]thymidine incorporation on the cell. The molecular weight of the purified protein was 10 kDa. The amino-terminal sequence of this 10-kDa protein was identical to bovine high mobility group protein (HMG) 1. This 10-kDa protein is suggested to be a basic protein and to have an HMG box, a consensus sequence motif among the HMG family. From these results, we named this protein HMG-like protein. HMG is a ubiquitous nonhistone component of chromatin and considered to be implicated in DNA replication. We found this protein in milk, and it showed a growth-promoting activity. We propose the possibility that HMG-like protein existed in milk and plays an important role for neonate in bone formation by activating osteoblasts.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cell Division / drug effects
  • Chromatography, High Pressure Liquid
  • Chromatography, Ion Exchange
  • Consensus Sequence / genetics
  • DNA / biosynthesis
  • High Mobility Group Proteins / chemistry
  • High Mobility Group Proteins / isolation & purification*
  • High Mobility Group Proteins / pharmacology*
  • Mice
  • Milk Proteins / chemistry*
  • Milk Proteins / pharmacology
  • Molecular Sequence Data
  • Molecular Weight
  • Osteoblasts / cytology*
  • Osteoblasts / drug effects
  • Sequence Alignment
  • Sequence Analysis
  • Tumor Cells, Cultured
  • Whey Proteins


  • High Mobility Group Proteins
  • Milk Proteins
  • Whey Proteins
  • DNA