Experimental induction of AGEs in fetal L132 lung cells changes the level of intracellular cathepsin D

Biochem Biophys Res Commun. 1999 Jul 22;261(1):175-82. doi: 10.1006/bbrc.1999.0902.

Abstract

The effect of the carbonyl compound glyoxal on the induction of advanced glycation end products (AGEs) in the fetal epithelial lung cells L132 was investigated using immunohistochemical, immunoelectron microscopic, and biochemical methods. It was found that glyoxal treatment resulted in morphological changes of the cells and in the membranous and cytosolic localization of AGEs such as methyl-glyoxal-derived compounds, N-(carboxymethyllysine) (CML) and imidazolone. The formation of AGEs was accompanied with a change in the intracellular expression of cathepsin D and a loss of enzymatic activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies
  • Cathepsin D / genetics
  • Cathepsin D / metabolism*
  • Cell Line
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cell Membrane / ultrastructure
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Cell Size / drug effects
  • Cytosol / drug effects
  • Cytosol / metabolism
  • Cytosol / ultrastructure
  • Epithelial Cells / drug effects
  • Epithelial Cells / enzymology
  • Epithelial Cells / metabolism
  • Epithelial Cells / ultrastructure
  • Gene Expression Regulation, Enzymologic* / drug effects
  • Glycation End Products, Advanced / metabolism*
  • Glyoxal / pharmacology
  • Humans
  • Imidazoles / metabolism
  • Immunohistochemistry
  • Lung / cytology
  • Lung / drug effects
  • Lung / embryology
  • Lung / metabolism*
  • Lysine / analogs & derivatives
  • Lysine / metabolism
  • Lysosomes / drug effects
  • Lysosomes / metabolism
  • Microscopy, Electron
  • Pulmonary Fibrosis / enzymology
  • Pulmonary Fibrosis / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • Antibodies
  • Glycation End Products, Advanced
  • Imidazoles
  • RNA, Messenger
  • imidazolone
  • Glyoxal
  • N(6)-carboxymethyllysine
  • Cathepsin D
  • Lysine