Identification of human rotavirus strains with the P[14] genotype by PCR

J Clin Microbiol. 1999 Aug;37(8):2706-8. doi: 10.1128/JCM.37.8.2706-2708.1999.

Abstract

A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid / genetics
  • Genome, Viral*
  • Humans
  • Polymerase Chain Reaction
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification