Isolation of an IgG monoclonal anti-dnaJ antibody from an immunoglobulin combinatorial library from a patient with rheumatoid arthritis

J Rheumatol. 1999 Jul;26(7):1439-45.


Objective: Previously, we showed that rheumatoid arthritis (RA) had both antibodies and T cells specific for the QKRAA-encompassing Escherichia coli dnaJ protein. These findings suggest that the bacteria induced anti-dnaJ responses may cross react with the human homolog of bacterial dnaJ in the joint, resulting in tissue damage.

Methods: We used the combinatorial library technique to isolate and characterize an IgG monoclonal anti-dnaJ antibody (designated CG1) from the blood of a patient with RA.

Results: Sequence analysis of CG1 revealed that its heavy and light chain V regions were respectively most homologous to the 3d279d VH4 and the O18 Vk1 genes. Interestingly, 3d279d is frequently expressed by B cells stimulated with staphylococcal enterotoxin; and O18 is the main gene employed by the Vk1 IgG antibodies against Haemophilus influenzae.

Conclusion: The combinatorial immunoglobulin library method represents an interesting model of how to approach the isolation and characterization of antibody-like reagents in the elucidation of autoantigens in RA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arthritis, Rheumatoid / genetics
  • Arthritis, Rheumatoid / immunology*
  • Bacterial Proteins / immunology*
  • Base Sequence
  • Escherichia coli / immunology*
  • Escherichia coli Proteins
  • Female
  • Gene Library
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins / immunology*
  • Humans
  • Immunoglobulin Fab Fragments / genetics
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology
  • Immunoglobulin G / isolation & purification*
  • Joints / immunology
  • Middle Aged
  • Molecular Sequence Data
  • Sequence Alignment
  • T-Lymphocytes / immunology


  • Bacterial Proteins
  • DnaJ protein, E coli
  • Escherichia coli Proteins
  • HSP40 Heat-Shock Proteins
  • Heat-Shock Proteins
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G