Immunohistochemical localization of connective tissue growth factor in the rat central nervous system

Brain Res. 1999 Jul 10;834(1-2):146-51. doi: 10.1016/s0006-8993(99)01517-6.


Connective tissue growth factor (CTGF) is an immediate early growth-responsive gene but its distribution and significance in the central nervous system (CNS) are unknown. We investigated the distribution of CTGF-like immunoreactivity (CTGF-IR) in the rat CNS using a specific antiserum against CTGF oligopeptide. The majority of CTGF-IR was observed in astrocytes. Ependymal cells lining the wall of the cerebral ventricle and tanycytes lining the central canal of the spinal cord showed the strongest CTGF-IR, while there was a diffuse but weak signal in the gray matter of the spinal cord. CTGF-IR was also detected in the cytoplasm of a subpopulation of pyramidal neurons in the cerebral cortex. Our results showed that CTGF-IR is widely distributed in the CNS at both regional and cellular levels, suggesting a complex functional role in the CNS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System / metabolism*
  • Cerebral Cortex / cytology
  • Cerebral Cortex / metabolism
  • Cerebral Ventricles / metabolism
  • Connective Tissue Growth Factor
  • Growth Substances / metabolism*
  • Immediate-Early Proteins*
  • Immunohistochemistry
  • Intercellular Signaling Peptides and Proteins*
  • Male
  • Pyramidal Cells / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord / cytology
  • Spinal Cord / metabolism
  • Tissue Distribution / physiology


  • CCN2 protein, rat
  • Growth Substances
  • Immediate-Early Proteins
  • Intercellular Signaling Peptides and Proteins
  • Connective Tissue Growth Factor