Generation of a Novel Polysaccharide by Inactivation of the aceP Gene From the Acetan Biosynthetic Pathway in Acetobacter Xylinum

Microbiology. 1999 Jun;145 ( Pt 6):1499-1506. doi: 10.1099/13500872-145-6-1499.


The acetan biosynthetic pathway in Acetobacter xylinum is an ideal model system for engineering novel bacterial polysaccharides. To genetically manipulate this pathway, an Acetobacter strain (CKE5), more susceptible to gene-transfer methodologies, was developed. A new gene, aceP, involved in acetan biosynthesis was identified, sequenced and shown to have homology at the amino acid level with beta-D-glucosyl transferases from a number of different organisms. Disruption of aceP in strain CKE5 confirmed the function assigned above and was used to engineer a novel polysaccharide with a pentasaccharide repeat unit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Carbohydrate Sequence
  • Genes, Bacterial*
  • Gluconacetobacter xylinus / enzymology
  • Gluconacetobacter xylinus / genetics
  • Gluconacetobacter xylinus / metabolism*
  • Glycosyltransferases / genetics*
  • Glycosyltransferases / isolation & purification
  • Glycosyltransferases / metabolism
  • Molecular Sequence Data
  • Polysaccharides, Bacterial / biosynthesis*
  • Polysaccharides, Bacterial / metabolism
  • Sequence Homology, Amino Acid
  • Transformation, Bacterial


  • Bacterial Proteins
  • Polysaccharides, Bacterial
  • acetan
  • AceP protein, Acetobacter xylinum
  • Glycosyltransferases

Associated data

  • GENBANK/Y18467