In vitro activation and repression of photosynthesis gene transcription in Rhodobacter capsulatus

Mol Microbiol. 1999 Jul;33(2):429-37. doi: 10.1046/j.1365-2958.1999.01490.x.

Abstract

It has been known for over half a century that anoxygenic photosynthetic bacteria maximally synthesize their photosystems in the absence of oxygen. During the last decade, it has become clear that this regulation is largely at the transcriptional level, with photosynthesis genes expressed only under anaerobic conditions. We describe here in vitro reconstitution of activation and repression of three photosynthesis promoters, bch (bacteriochlorophyll biosynthesis), puc (light-harvesting II apoproteins) and puf (reaction centre and light-harvesting I apoproteins) using purified transcription factors and RNA polymerase from Rhodobacter capsulatus. Previous genetic results have indicated that each of these three promoters is differentially regulated by three key regulators: CrtJ acting as a repressor of bch and puc and the two-component regulators RegA/RegB, which are activators of puc and puf. These regulators are distinct from those that mediate oxygen control in enteric bacteria. Our in vitro studies show that these purified regulators directly control the expression of the housekeeping RNA polymerase at these promoters. High-level basal expression of the bch promoter is shown to be repressed by CrtJ. The puc promoter is activated by the RegB-phosphorylated RegA protein and additionally repressed by CrtJ. At the puc promoter, CrtJ effectively competes for promoter binding with RegA, while at the bch promoter, repression appears to be by competition for the RNA polymerase binding site. In contrast to what has been suggested previously, the RegA-activated puf promoter is demonstrated as being recognized by the housekeeping RNA polymerase. We also discuss evidence that RegA approximately P activation of the puc and puf promoters involves recruitment of RNA polymerase by different modes of protein-protein interaction.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • Base Sequence
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Gene Expression Regulation, Bacterial*
  • Light-Harvesting Protein Complexes
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / genetics
  • Photosynthesis / genetics*
  • Photosynthetic Reaction Center Complex Proteins / metabolism
  • Promoter Regions, Genetic
  • Protein Kinases*
  • Rhodobacter capsulatus / genetics*
  • Trans-Activators / metabolism
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Light-Harvesting Protein Complexes
  • Photosynthetic Reaction Center Complex Proteins
  • RegA protein, Rhodobacter
  • RegB protein, Rhodobacter
  • Trans-Activators
  • Protein Kinases
  • Phosphoprotein Phosphatases