Effects of interferons on proliferation and collagen synthesis of rat palatal wound fibroblasts

Arch Oral Biol. 1999 Jul;44(7):541-7. doi: 10.1016/s0003-9969(99)00037-0.


The purpose was to select drugs that specifically reduce collagen synthesis by palatal granulation fibroblasts without affecting their proliferation. Granulation fibroblasts were obtained from 8-day-old palatal mucoperiosteal wounds and normal fibroblasts from palatal tissue of unwounded rats. Cultured cells were treated with interferon-alpha2b, interferon-beta and interferon-gamma (0, 100, 1000, and 10000 U/ml). Cell proliferation was measured by [3H]thymidine incorporation. Collagen synthesis and non-collagenous protein synthesis were determined from the incorporation of [3H]proline. None of the interferons significantly inhibited the proliferation of either type of fibroblasts. Interferon-alpha2b had no effect on the variables studied at the dosages used. Interferon-beta reduced collagen synthesis of granulation fibroblasts without affecting their non-collagenous protein synthesis or protein synthesis by normal fibroblasts. Interferon-gamma reduced collagen synthesis of both types of fibroblast and the non-collagenous protein synthesis of granulation fibroblasts. These data show that interferon-beta specifically reduces collagen synthesis by oral granulation fibroblasts without affecting normal palatal fibroblasts.

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Cells, Cultured
  • Collagen / biosynthesis*
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Granulation Tissue / cytology
  • Granulation Tissue / drug effects
  • Granulation Tissue / metabolism
  • Interferon alpha-2
  • Interferon-alpha / pharmacology*
  • Interferon-beta / pharmacology*
  • Interferon-gamma / pharmacology*
  • Male
  • Mouth Mucosa / cytology
  • Mouth Mucosa / drug effects
  • Mouth Mucosa / injuries
  • Mouth Mucosa / metabolism
  • Palate / cytology
  • Palate / drug effects*
  • Palate / injuries
  • Palate / metabolism
  • Protein Biosynthesis
  • Rats
  • Rats, Wistar
  • Recombinant Proteins
  • Wound Healing / drug effects


  • Interferon alpha-2
  • Interferon-alpha
  • Recombinant Proteins
  • Interferon-beta
  • Interferon-gamma
  • Collagen