We describe the expression pattern of cSix4, a chick homologue of the murine Six4/AREC3 gene. cSix4 transcripts are detected at gastrula stages in the blastoderm surrounding the developing axial midline. As the neural plate begins to form cSix4 mRNA is detected in a crescent-shaped band, which surrounds the anterior developing neural plate and corresponds to the presumptive placode region. This expression is maintained in all the placodes (olfactory, optic, neural and otic) as they develop but with different characteristics. Further, abundant expression of cSix4 was localised to the paraxial mesoderm and the entire developing somites, becoming restricted first to their dorsal portion, then to the dermomyotome and finally to the myotome. cSix4 expression is maintained in the developing and adult muscular tissue. Additional sites of cSix4 expression are the presumptive and developing limb buds, the notochord, trigeminal ganglia, cells of the spinal cord, particularly the motor neurones, the dorsal root ganglia, the neural retina, as well as the epithelial component of the developing kidney.