Determination of unbound drug concentration and protein-drug binding fraction in plasma

Biomed Chromatogr. 1999 Jun;13(4):262-6. doi: 10.1002/(SICI)1099-0801(199906)13:4<262::AID-BMC832>3.0.CO;2-2.


The aim of this work was to provide a short overview of existing methods for the determination of free drug concentration and protein-drug binding fraction in plasma. Various methods have been described in terms of principles, evaluation of methods, and applications in recent years, with an emphasis on the chromatographic method, i.e. high-performance frontal analysis (HPFA).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Proteins / metabolism*
  • Chromatography, Gel / methods
  • Chromatography, High Pressure Liquid
  • Humans
  • Microdialysis
  • Pharmaceutical Preparations / metabolism*
  • Pharmacokinetics
  • Protein Binding
  • Ultrafiltration


  • Blood Proteins
  • Pharmaceutical Preparations