Differential distribution of T lymphocyte subpopulations in the bovine mammary gland during lactation

J Dairy Sci. 1999 Jul;82(7):1459-64. doi: 10.3168/jds.S0022-0302(99)75373-7.

Abstract

The existence and distribution of T lymphocyte subpopulations in the mammary parenchymal tissue of cows were immunohistochemically detailed during early lactation. CD2+, CD4+, and CD8+ T lymphocytes were localized primarily in the mammary parenchymal tissue. CD8+ T lymphocytes were predominant over CD4+ T lymphocytes and occurred in close contact with the alveolar epithelium and between epithelial cells in the central area of the upper mammary gland. CD4+ T lymphocytes were present in equal numbers in the epithelial and connective tissue area. Occasionally, both CD4+ and CD8+ T lymphocytes formed cell clusters in the interalveolar connective tissue. The ratio of CD4+ T lymphocytes to CD8+ T lymphocytes was less than 1.0 and was lower in the epithelial area than in the connective tissue. The distribution of CD4+ and CD8+ T lymphocytes was similar in the parenchymal tissue of the gland cistern. The observation that there is a preferential presence of CD8+ T lymphocytes in the epithelial area of the bovine mammary gland during early lactation might indicate that these cells participate in the maintenance of the integrity of the epithelium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte / analysis
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • Cattle
  • Connective Tissue / immunology
  • Epithelium / immunology
  • Female
  • Lactation / immunology*
  • Mammary Glands, Animal / cytology
  • Mammary Glands, Animal / immunology*
  • T-Lymphocyte Subsets / cytology*
  • T-Lymphocyte Subsets / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte