A different mode of entry by dengue-2 neutralisation escape mutant virus

Arch Virol. 1999;144(5):989-95. doi: 10.1007/s007050050561.

Abstract

Entry processes were compared between dengue-2 (DEN-2) virus and a DEN-2 neutralisation escape mutant virus in baby hamster kidney (BHK) cells. The mutant virus (mu6B2) was resistant to neutralisation by a monoclonal antibody, MAb 6B2. Infection of BHK cells by the wild-type DEN-2 virus resulted in direct penetration of the virions into the cytoplasm whereas the mutant virus entered cells by endocytosis. The continual presence of the monoclonal antibody might have asserted some pressure for the mutant to adopt a different mode of entry.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Cell Line
  • Cell Membrane / ultrastructure
  • Cell Membrane / virology
  • Cricetinae
  • Cryoelectron Microscopy
  • Cytoplasm / virology
  • Dengue Virus / genetics
  • Dengue Virus / physiology*
  • Dengue Virus / ultrastructure
  • Endocytosis
  • Kidney
  • Neutralization Tests
  • Virion / genetics
  • Virion / physiology
  • Virion / ultrastructure

Substances

  • Antibodies, Monoclonal