Molecular and functional regulation of two NO3- uptake systems by N- and C-status of Arabidopsis plants

Plant J. 1999 Jun;18(5):509-19. doi: 10.1046/j.1365-313x.1999.00480.x.

Abstract

Root NO3- uptake and expression of two root NO3- transporter genes (Nrt2;1 and Nrt1) were investigated in response to changes in the N- or C-status of hydroponically grown Arabidopsis thaliana plants. Expression of Nrt2;1 is up-regulated by NO3 - starvation in wild-type plants and by N-limitation in a nitrate reductase (NR) deficient mutant transferred to NO3- as sole N source. These observations show that expression of Nrt2;1 is under feedback repression by N-metabolites resulting from NO3- reduction. Expression of Nrt1 is not subject to such a repression. However, Nrt1 is over-expressed in the NR mutant even under N-sufficient conditions (growth on NH4NO3 medium), suggesting that expression of this gene is affected by the presence of active NR, but not by N-status of the plant. Root 15NO3- influx is markedly increased in the NR mutant as compared to the wild-type. Nevertheless, both genotypes have similar net 15NO3- uptake rates due to a much larger 14NO3- efflux in the mutant than in the wild-type. Expressions of Nrt2;1 and Nrt1 are diurnally regulated in photosynthetically active A. thaliana plants. Both increase during the light period and decrease in the first hours of the dark period. Sucrose supply prevents the inhibition of Nrt2;1 and Nrt1 expressions in the dark. In all conditions investigated, Nrt2;1 expression is strongly correlated with root 15NO3- influx at 0.2 mM external concentration. In contrast, changes in the Nrt1 mRNA level are not always associated with similar changes in the activities of high- or low-affinity NO3- transport systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Biological
  • Anion Transport Proteins*
  • Arabidopsis / physiology
  • Arabidopsis Proteins*
  • Biological Transport, Active
  • Carbon / deficiency
  • Carrier Proteins / biosynthesis*
  • Circadian Rhythm
  • Culture Media
  • Gene Expression Regulation, Plant*
  • Genotype
  • Nitrates / metabolism*
  • Nitrogen / deficiency
  • Plant Proteins*
  • Plant Roots / physiology*
  • Sucrose / pharmacology
  • Up-Regulation

Substances

  • Anion Transport Proteins
  • Arabidopsis Proteins
  • Carrier Proteins
  • Culture Media
  • NRT1.1 protein, Arabidopsis
  • NRT2 protein, Arabidopsis
  • Nitrates
  • Plant Proteins
  • nitrate transporters
  • Sucrose
  • Carbon
  • Nitrogen