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. 1999 Jun 30;67(3):221-30.
doi: 10.1016/s0378-1135(99)00042-5.

Characterization of Bovine Coronavirus Isolates/From Eight Different States in the USA

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Free PMC article

Characterization of Bovine Coronavirus Isolates/From Eight Different States in the USA

S Kapil et al. Vet Microbiol. .
Free PMC article

Abstract

Bovine coronavirus isolates from eight different states of the USA were compared for their antigenic properties and susceptibility to hygromycin B. Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focusing among viral proteins with isoelectric points between 4.45-4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5 mM) whereas a few hygromycin B resistant isolates were also found.

Figures

Fig. 1
Fig. 1
Hemagglutination-inhibition (HI) activity of an anti-bovine coronavirus (Mebus isolate). Antiserum against wild-type bovine coronavirus isolates: X-axis denotes the HI titers and the numbers indicate superscripts (Log 2). On the basis of a one-way HI test, only 4 out of 44 isolates were inhibited up to 1 : 1024–4096, Type I; 1 was inhibited up to 1 : 128, Type II; 30 were inhibited up to 1 : 16 and 9 isolates were not inhibited, Type III. Y-axis indicates number of BCV isolates inhibited at a particular HI titer.
Fig. 2
Fig. 2
Hemagglutination-inhibition activity of an anti-bovine coronavirus (Mebus isolate). Antiserum against cell culture propagated bovine coronavirus isolates: on the basis of a one way HI test, 77 BCV isolates were inhibited from 1 : 2 up to 1 : 1024. X-axis denotes the HI titers and the numbers indicate superscripts (Log 2). Y-axis indicates number of BCV isolates inhibited.
Fig. 3
Fig. 3
Polypeptide profile of bovine coronavirus by isoelectric focusing: pI of the proteins was calculated by comparing with a IEF standard pI 4.45–8.0 (Bio Rad). A double band towards the anodic end of the gel was found to differ (either 4.45 to 4.65; shown with arrows) between different isolates and different fractions of the same isolate.

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