Biodistribution of an adenoviral vector carrying the luciferase reporter gene following intravesical or intravenous administration to a mouse

Cancer Gene Ther. Jul-Aug 1999;6(4):367-72. doi: 10.1038/sj.cgt.7700090.

Abstract

The biodistribution and resulting pattern of transgene expression were determined following intravesical administration of an adenoviral vector carrying the luciferase reporter gene (AdLuc). Female BALB/c mice were subjected to intravesical instillation of 1 x 10(9) or 5 x 10(9) plaque-forming units of AdLuc. After sacrifice, transgene expression was detected in tissues using luciferase assays; vector DNA was detected by vector-specific polymerase chain reaction. These experiments showed very little vector dissemination outside of the bladder by this route of administration. High-level expression of the vector transgene in the bladder was found to diminish by severalfold after 3 days. In a supporting study, vector dissemination and resulting transgene expression were determined following tail vein injection of 5 x 10(9) plaque-forming units of AdLuc. Vector was distributed to and expressed in every organ analyzed, with the highest concentration and level of expression observed in the liver.

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Female
  • Genes, Reporter*
  • Genetic Vectors / administration & dosage
  • Genetic Vectors / pharmacokinetics*
  • Injections, Intravenous
  • Luciferases / genetics*
  • Mice
  • Mice, Inbred BALB C
  • Pilot Projects
  • Polymerase Chain Reaction
  • Tissue Distribution
  • Urinary Bladder / enzymology

Substances

  • Luciferases