Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei

D DeShazer; P J Brett; M N Burtnick; D E Woods

doi:10.1128/JB.181.15.4661-4664.1999

Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei

J Bacteriol. 1999 Aug;181(15):4661-4. doi: 10.1128/JB.181.15.4661-4664.1999.

Authors

D DeShazer¹, P J Brett, M N Burtnick, D E Woods

Affiliation

¹ Genetics and Physiology Branch, Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702, USA.

Abstract

Previous studies have demonstrated that Burkholderia pseudomallei secretes protease, lipase, and phospholipase C (PLC) into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis have not been elucidated. In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC.

MeSH terms

Burkholderia pseudomallei / enzymology*
Burkholderia pseudomallei / genetics*
Chromosome Mapping
Chromosomes, Bacterial
Endopeptidases / biosynthesis
Endopeptidases / genetics*
Escherichia coli / genetics
Genes, Bacterial*
Lipase / biosynthesis
Lipase / genetics*
Molecular Sequence Data
Multigene Family*
Mutagenesis, Insertional
Plasmids
Type C Phospholipases / biosynthesis
Type C Phospholipases / genetics*

Substances

Lipase
Type C Phospholipases
Endopeptidases

Associated data

GENBANK/AF110185
GENBANK/AF110186