Abstract
The TPS1 gene from Hansenula polymorpha, which encodes trehalose-6-phosphate (Tre6P) synthase, has been isolated and characterized. The deletion of TPS1 rendered H. polymorpha cells incapable of trehalose synthesis under conditions where wild-type cells normally accumulate high levels of trehalose. Interestingly, the loss of Tre6P synthase did not cause any obvious growth defects on a glucose-containing medium, even at high temperatures, but seriously compromised the cells' ability to acquire thermotolerance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Gene Deletion
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Genetic Complementation Test
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Glucosyltransferases / genetics
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Glucosyltransferases / metabolism*
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Hot Temperature
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Kinetics
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Pichia / enzymology
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Pichia / genetics
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Pichia / growth & development*
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Saccharomyces cerevisiae / genetics
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Schizosaccharomyces / genetics
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Sugar Phosphates / metabolism
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Temperature
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Trehalose / analogs & derivatives
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Trehalose / biosynthesis*
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Trehalose / metabolism
Substances
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Sugar Phosphates
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trehalose-6-phosphate
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Trehalose
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Glucosyltransferases
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trehalose-6-phosphate synthase