The thermophilic yeast Hansenula polymorpha does not require trehalose synthesis for growth at high temperatures but does for normal acquisition of thermotolerance

J Bacteriol. 1999 Aug;181(15):4665-8. doi: 10.1128/JB.181.15.4665-4668.1999.

Abstract

The TPS1 gene from Hansenula polymorpha, which encodes trehalose-6-phosphate (Tre6P) synthase, has been isolated and characterized. The deletion of TPS1 rendered H. polymorpha cells incapable of trehalose synthesis under conditions where wild-type cells normally accumulate high levels of trehalose. Interestingly, the loss of Tre6P synthase did not cause any obvious growth defects on a glucose-containing medium, even at high temperatures, but seriously compromised the cells' ability to acquire thermotolerance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Deletion
  • Genetic Complementation Test
  • Glucosyltransferases / genetics
  • Glucosyltransferases / metabolism*
  • Hot Temperature
  • Kinetics
  • Pichia / enzymology
  • Pichia / genetics
  • Pichia / growth & development*
  • Saccharomyces cerevisiae / genetics
  • Schizosaccharomyces / genetics
  • Sugar Phosphates / metabolism
  • Temperature
  • Trehalose / analogs & derivatives
  • Trehalose / biosynthesis*
  • Trehalose / metabolism

Substances

  • Sugar Phosphates
  • trehalose-6-phosphate
  • Trehalose
  • Glucosyltransferases
  • trehalose-6-phosphate synthase

Associated data

  • GENBANK/AJ010725