Dally Cooperates With Drosophila Frizzled 2 to Transduce Wingless Signalling

Nature. 1999 Jul 15;400(6741):281-4. doi: 10.1038/22343.

Abstract

The Drosophila wingless gene (wg) encodes a protein of the Wnt family and is a critical regulator in many developmental processes. Biochemical studies have indicated that heparan sulphate proteoglycans, consisting of a protein core to which heparan sulphate glycosaminoglycans are attached, are important for Wg function. Here we show that, consistent with these findings, the Drosophila gene sulfateless (sfl), which encodes a homologue of vertebrate heparan sulphate N-deacetylase/N-sulphotransferase (an enzyme needed for the modification of heparan sulphate) is essential for Wg signalling. We have identified the product of division abnormally delayed (dally), a glycosyl-phosphatidyl inositol (GPI)-linked glypican, as a heparan sulphate proteoglycan molecule involved in Wg signalling. Our results indicate that Dally may act as a co-receptor for Wg, and that Dally, together with Drosophila Frizzled 2, modulates both short- and long-range activities of Wg.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amidohydrolases / genetics
  • Amidohydrolases / physiology
  • Amino Acid Sequence
  • Animals
  • Body Patterning
  • Drosophila / embryology
  • Drosophila / genetics
  • Drosophila / physiology*
  • Drosophila Proteins*
  • Female
  • Frizzled Receptors
  • Genes, Insect
  • Heparin / analogs & derivatives
  • Heparin / physiology
  • Insect Proteins / genetics
  • Insect Proteins / physiology
  • Membrane Glycoproteins / physiology*
  • Molecular Sequence Data
  • Mutation
  • Proteoglycans / physiology*
  • Proto-Oncogene Proteins / physiology*
  • Receptors, G-Protein-Coupled
  • Receptors, Neurotransmitter / physiology*
  • Sequence Homology, Amino Acid
  • Signal Transduction*
  • Sulfotransferases / genetics
  • Sulfotransferases / physiology
  • Wings, Animal / embryology
  • Wnt1 Protein

Substances

  • Drosophila Proteins
  • Frizzled Receptors
  • Insect Proteins
  • Membrane Glycoproteins
  • Proteoglycans
  • Proto-Oncogene Proteins
  • Receptors, G-Protein-Coupled
  • Receptors, Neurotransmitter
  • Wnt1 Protein
  • dally protein, Drosophila
  • fz2 protein, Drosophila
  • heparin proteoglycan
  • wg protein, Drosophila
  • Heparin
  • Sulfotransferases
  • heparitin sulfotransferase
  • sfl protein, Drosophila
  • Amidohydrolases

Associated data

  • GENBANK/AF175689