Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24

Appl Microbiol Biotechnol. 1999 Jun;51(6):786-93. doi: 10.1007/s002530051463.


Rhodococcus strain 124 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We have identified a cosmid clone from 124 genomic DNA that is able to confer the ability to convert indene to indandiol upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalenetype dioxygenase. DNA sequence analysis of a portion of this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase. These genes will be useful for manipulating indene bioconversion in Rhodococcus strain 124.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Chromatography, High Pressure Liquid
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Gene Library
  • Genes, Bacterial*
  • Indenes / metabolism*
  • Molecular Sequence Data
  • Oxidoreductases / genetics
  • Oxidoreductases / metabolism
  • Oxygenases / genetics*
  • Oxygenases / metabolism
  • Phylogeny
  • Plasmids
  • Rhodococcus / enzymology
  • Rhodococcus / genetics*
  • Sequence Analysis, DNA


  • DNA, Bacterial
  • Indenes
  • Oxidoreductases
  • Oxygenases

Associated data

  • GENBANK/AF121905