Extensive 16S rRNA gene sequence diversity in Campylobacter hyointestinalis strains: taxonomic and applied implications

Int J Syst Bacteriol. 1999 Jul;49 Pt 3:1171-5. doi: 10.1099/00207713-49-3-1171.

Abstract

Phylogenetic relationships of Campylobacter hyointestinalis subspecies were examined by means of 16S rRNA gene sequencing. Sequence similarities among C. hyointestinalis subsp. lawsonii strains exceeded 99.0%, but values among C. hyointestinalis subsp. hyointestinalis strains ranged from 96.4 to 100%. Sequence similarities between strains representing the two different subspecies ranged from 95.7 to 99.0%. An intervening sequence was identified in certain of the C. hyointestinalis subsp. lawsonii strains. C. hyointestinalis strains occupied two distinct branches in a phylogenetic analysis of the genus Campylobacter, emphasizing the need for multiple strain analysis when using 16S rRNA gene sequence comparisons for taxonomic investigations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Campylobacter / classification*
  • Campylobacter / genetics*
  • Campylobacter Infections / microbiology
  • Campylobacter Infections / veterinary
  • Cattle
  • Cattle Diseases / microbiology
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Diarrhea / veterinary
  • Genes, rRNA*
  • Genetic Variation*
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics*
  • Sequence Analysis, DNA
  • Stomach / microbiology
  • Swine
  • Swine Diseases / microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AF097681
  • GENBANK/AF097682
  • GENBANK/AF097683
  • GENBANK/AF097684
  • GENBANK/AF097685
  • GENBANK/AF097686
  • GENBANK/AF097687
  • GENBANK/AF097688
  • GENBANK/AF097689
  • GENBANK/AF097690
  • GENBANK/AF097691