IL-9 induces chemokine expression in lung epithelial cells and baseline airway eosinophilia in transgenic mice

Eur J Immunol. 1999 Jul;29(7):2130-9. doi: 10.1002/(SICI)1521-4141(199907)29:07<2130::AID-IMMU2130>3.0.CO;2-S.


Recent data have identified IL-9 as a key cytokine in determining susceptibility to asthma. These data are supported by the finding that allergen-exposed IL-9-transgenic mice exhibit many features that are characteristic of human asthma (airway eosinophilia, elevated serum IgE and bronchial hyperresponsiveness) as compared to the background strain. A striking feature of these animals is a robust peribronchial and perivascular eosinophilia after allergen challenge, suggesting that IL-9 is a potent factor in regulating this process. In an attempt to gain insights into the molecular mechanism governing IL-9 modulation of lung eosinophilia, we investigated the ability of this cytokine to induce the expression of CC-type chemokines in the lung because of their effect on stimulating eosinophil chemotaxis. Here we show that IL-9-transgenic mice in contrast to their congenic controls exhibit baseline lung eosinophilia that is associated with the up-regulation of CC-chemokine expression in the airway. This effect appears to be through a direct action of IL-9 because the addition of recombinant IL-9 to primary epithelial cultures and cell lines induced the expression of these chemokines in vitro. These data support a mechanism for IL-9 in regulating the expression of eosinophil chemotactic factors in lung epithelial cells.

MeSH terms

  • Allergens / administration & dosage
  • Animals
  • Asthma / etiology
  • Asthma / immunology
  • Base Sequence
  • Cell Line
  • Cells, Cultured
  • Chemokines / biosynthesis*
  • Chemokines / genetics
  • Chemotaxis, Leukocyte
  • DNA Primers / genetics
  • Eosinophilia / etiology*
  • Eosinophilia / immunology
  • Epithelial Cells / immunology
  • Humans
  • Interleukin-9 / genetics*
  • Interleukin-9 / pharmacology
  • Interleukin-9 / physiology*
  • Lung / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Molecular Sequence Data
  • Up-Regulation


  • Allergens
  • Chemokines
  • DNA Primers
  • Interleukin-9

Associated data

  • GENBANK/AA178071
  • GENBANK/J04467
  • GENBANK/M33266
  • GENBANK/M35590
  • GENBANK/M73061
  • GENBANK/M84746
  • GENBANK/S71251
  • GENBANK/U40672
  • GENBANK/U66670