Cloning and heterologous expression of a cDNA encoding 1-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana

FEBS Lett. 1999 Jul 16;455(1-2):140-4. doi: 10.1016/s0014-5793(99)00849-2.


Various plant isoprenoids are synthesized via the non-mevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP) by an enzyme which was recently cloned from Escherichia coli. In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) we cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin specifically inhibits this DXR enzyme activity.

MeSH terms

  • Aldose-Ketose Isomerases / genetics*
  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • Escherichia coli / genetics
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Oxidoreductases / genetics*
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Multienzyme Complexes
  • Oxidoreductases
  • 1-deoxy-D-xylulose 5-phosphate reductoisomerase
  • Aldose-Ketose Isomerases

Associated data

  • GENBANK/AJ242588