Expression of macrophage inflammatory protein-1alpha (MIP-1alpha) in human endometrium throughout the menstrual cycle

Br J Obstet Gynaecol. 1999 Jul;106(7):725-30. doi: 10.1111/j.1471-0528.1999.tb08374.x.


Objective: To investigate the distribution and the role of macrophage inflammatory protein-1alpha (MIP-1alpha) in human endometrium during cyclic changes.

Setting: Department of Obstetrics and Gynaecology, Shiga University of Medical Science and University Hospital.

Materials: Eighteen endometrial tissue specimens surgically resected or biopsied from women with normal menstrual cycles, without hormonal disorder or endometrial diseases.

Methods: By immunohistochemistry, using monoclonal antibodies (lambda delta 78 for MIP-1alpha and CR3/43 for human leukocyte antigen-DR (HLA-DR)).

Results: Immunoreactivity for anti-MIP-1alpha was distributed diffusely in epithelial cells throughout the proliferative and secretory phases but was absent during menstruation due to degenerative or necrotic changes. HLA-DR was expressed in epithelial cells only in the late secretory phase and was not expressed in stromal cells.

Conclusion: Immunohistochemical analysis showed the presence of MIP-1alpha in the endometrial epithelium. Expression of HLA-DR in epithelial cells was observed only in the late secretory phase, suggesting that accumulation of MIP-1alpha in epithelium occurred by self production and not via a receptor mediated pathway. MIP-1alpha was released from the denuded epithelium during menstruation and appeared to contribute to the accumulation of monocytes/macrophages into the endometrial cavity. MIP-1alpha has a number of biological effects other than monocytic chemotaxis, and some of these effects may be exerted in the endometrial tissue.

MeSH terms

  • Biomarkers
  • Chemokine CCL3
  • Chemokine CCL4
  • Endometrium / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • Macrophage Inflammatory Proteins / metabolism*
  • Menstrual Cycle / metabolism*


  • Biomarkers
  • Chemokine CCL3
  • Chemokine CCL4
  • Macrophage Inflammatory Proteins